Automated assessment of bone changes in cross-sectional micro-CT studies of murine experimental osteoarthritis.

ABSTRACT:

Objective

The degradation of articular cartilage, which characterises osteoarthritis (OA), is usually paired with excessive bone remodelling, including subchondral bone sclerosis, cysts, and osteophyte formation. Experimental models of OA are widely used to investigate pathogenesis, yet few validated methodologies for assessing periarticular bone morphology exist and quantitative measurements are limited by manual segmentation of micro-CT scans. The aim of this work was to chart the temporal changes in periarticular bone in murine OA by novel, automated micro-CT methods.

Methods

OA was induced by destabilisation of the medial meniscus (DMM) in 10-week old male mice and disease assessed cross-sectionally from 1- to 20-weeks post-surgery. A novel approach was developed to auto

SUBMITTER: Das Neves Borges P 

PROVIDER: S-EPMC5363908 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature