Calcium Directly Regulates Phosphatidylinositol 4,5-Bisphosphate Headgroup Conformation and Recognition.

ABSTRACT: The orchestrated recognition of phosphoinositides and concomitant intracellular release of Ca²⁺ is pivotal to almost every aspect of cellular processes, including membrane homeostasis, cell division and growth, vesicle trafficking, as well as secretion. Although Ca²⁺ is known to directly impact phosphoinositide clustering, little is known about the molecular basis for this or its significance in cellular signaling. Here, we study the direct interaction of Ca²⁺ with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂), the main lipid marker of the plasma membrane. Electrokinetic potential measurements of PI(4,5)P₂ containing liposomes reveal that Ca²⁺ as well as Mg²⁺ reduce the zeta potential of liposomes to nearly ba

SUBMITTER: Bilkova E 

PROVIDER: S-EPMC5364432 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature