Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system.

ABSTRACT: Despite major advances on miRNA profiling and target predictions, functional readouts for endogenous miRNAs are limited and frequently lead to contradicting conclusions. Numerous approaches including functional high-throughput and miRISC complex evaluations suggest that the functional miRNAome differs from the predictions based on quantitative sRNA profiling. To resolve the apparent contradiction of expression versus function, we generated and applied a fluorescence reporter gene assay enabling single cell analysis. This approach integrates and adapts a mathematical model for miRNA-driven gene repression. This model predicts three distinct miRNA-groups with unique repression activities (low, mid and high) governed not just by expression levels but also by miRNA/target-binding capability. H

SUBMITTER: Lemus-Diaz N 

PROVIDER: S-EPMC5364550 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature