Widespread promoter methylation of synaptic plasticity genes in long-term potentiation in the adult brain in vivo.

ABSTRACT:

Background

DNA methylation is a key modulator of gene expression in mammalian development and cellular differentiation, including neurons. To date, the role of DNA modifications in long-term potentiation (LTP) has not been explored.

Results

To investigate the occurrence of DNA methylation changes in LTP, we undertook the first detailed study to describe the methylation status of all known LTP-associated genes during LTP induction in the dentate gyrus of live rats. Using a methylated DNA immunoprecipitation (MeDIP)-array, together with previously published matched RNA-seq and public histone modification data, we discover widespread changes in methylation status of LTP-genes. We further show that the expression of many LTP-genes is correlated with their methylation status. We

SUBMITTER: Maag JL 

PROVIDER: S-EPMC5364592 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature