Genome-wide expression profiling establishes novel modulatory roles of vitamin C in THP-1 human monocytic cell line.

ABSTRACT:

Background

Vitamin C (vit C) is an essential dietary nutrient, which is a potent antioxidant, a free radical scavenger and functions as a cofactor in many enzymatic reactions. Vit C is also considered to enhance the immune effector function of macrophages, which are regarded to be the first line of defence in response to any pathogen. The THP-1 cell line is widely used for studying macrophage functions and for analyzing host cell-pathogen interactions.

Results

We performed a genome-wide temporal gene expression and functional enrichment analysis of THP-1 cells treated with 100 μM of vit C, a physiologically relevant concentration of the vitamin. Modulatory effects of vitamin C on THP-1 cells were revealed by differential expression of genes starting from 8 h onwards. The num

SUBMITTER: Batra SD 

PROVIDER: S-EPMC5364625 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature