Inhibition of the β-Lactamase Bla_Mab by Avibactam Improves the In Vitro and In Vivo Efficacy of Imipenem against Mycobacterium abscessus.

ABSTRACT: Mycobacterium abscessus pulmonary infections are treated with a macrolide (clarithromycin or azithromycin), an aminoglycoside (amikacin), and a β-lactam (cefoxitin or imipenem). The triple combination is used without any β-lactamase inhibitor, even though Mabscessus produces the broad-spectrum β-lactamase Bla_Mab We determine whether inhibition of Bla_Mab by avibactam improves the activity of imipenem against M. abscessus The bactericidal activity of drug combinations was assayed in broth and in human macrophages. The in vivo efficacy of the drugs was tested by monitoring the survival of infected zebrafish embryos. The level of Bla_Mab production in broth and in macrophages was compared by quantitative reverse transcription-P

SUBMITTER: Lefebvre AL 

PROVIDER: S-EPMC5365697 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature