Fixation methods can differentially affect ciliary protein immunolabeling.

ABSTRACT:

Background

Primary cilia are immotile, microtubule-based organelles present on most cells. Defects in primary cilia presence/function result in a category of developmental diseases referred to as ciliopathies. As the cilia field progresses, there is a need to consider both the ciliary and extraciliary roles of cilia proteins. However, traditional fixation methods are not always suitable for examining the full range of localizations of cilia proteins. Here, we tested a variety of fixation methods with commonly used cilia markers to determine the most appropriate fixation method for different cilia proteins.

Methods

Mouse inner medullary collecting duct and human retinal pigmented epithelial cells were grown to confluence, serum starved, and fixed with one of the following fix

SUBMITTER: Hua K 

PROVIDER: S-EPMC5366141 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature