A primary human macrophage-enteroid co-culture model to investigate mucosal gut physiology and host-pathogen interactions.

ABSTRACT: Integration of the intestinal epithelium and the mucosal immune system is critical for gut homeostasis. The intestinal epithelium is a functional barrier that secludes luminal content, senses changes in the gut microenvironment, and releases immune regulators that signal underlying immune cells. However, interactions between epithelial and innate immune cells to maintain barrier integrity and prevent infection are complex and poorly understood. We developed and characterized a primary human macrophage-enteroid co-culture model for in-depth studies of epithelial and macrophage interactions. Human intestinal stem cell-derived enteroid monolayers co-cultured with human monocyte-derived macrophages were used to evaluate barrier function, cytokine secretion, and protein expression under basal c

SUBMITTER: Noel G 

PROVIDER: S-EPMC5366908 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature