Time- and dose dependent actions of cardiotonic steroids on transcriptome and intracellular content of Na⁺ and K⁺: a comparative analysis.

ABSTRACT: Recent studies demonstrated that in addition to Na⁺,K⁺-ATPase inhibition cardiotonic steroids (CTSs) affect diverse intracellular signaling pathways. This study examines the relative impact of [Na⁺]_i/[K⁺]_i-mediated and -independent signaling in transcriptomic changes triggered by the endogenous CTSs ouabain and marinobufagenin (MBG) in human umbilical vein endothelial cells (HUVEC). We noted that prolongation of incubation increased the apparent affinity for ouabain estimated by the loss of [K⁺]_i and gain of [Na⁺]_i. Six hour exposure of HUVEC to 100 and 3,000 nM ouabain resulted in elevation of the [Na⁺]_i/[K⁺]_i ratio by ~15 and 80-fold an

SUBMITTER: Klimanova EA 

PROVIDER: S-EPMC5366943 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature