Role of inducible nitric oxide synthase in endothelium-independent relaxation to raloxifene in rat aorta.

ABSTRACT:

Background and purpose

Raloxifene can induce both endothelium-dependent and -independent relaxation in different arteries. However, the underlying mechanisms by which raloxifene triggers endothelium-independent relaxation are still incompletely understood. The purpose of present study was to examine the roles of NOSs and Ca²⁺ channels in the relaxant response to raloxifene in the rat isolated, endothelium-denuded aorta.

Experimental approach

Changes in isometric tension, cGMP, nitrite, inducible NOS protein expression and distribution in response to raloxifene in endothelium-denuded aortic rings were studied by organ baths, radioimmunoassay, Griess reaction, western blot and immunohistochemistry respectively.

Key results

Raloxifene reduced the contraction t

SUBMITTER: Wong CM 

PROVIDER: S-EPMC5368050 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature