Differential dehydration effects on globular proteins and intrinsically disordered proteins during film formation.

ABSTRACT: Globular proteins composed of different secondary structures and fold types were examined by synchrotron radiation circular dichroism spectroscopy to determine the effects of dehydration on their secondary structures. They exhibited only minor changes upon removal of bulk water during film formation, contrary to previously reported studies of proteins dehydrated by lyophilization (where substantial loss of helical structure and gain in sheet structure was detected). This near lack of conformational change observed for globular proteins contrasts with intrinsically disordered proteins (IDPs) dried in the same manner: the IDPs, which have almost completely unordered structures in solution, exhibited increased amounts of regular (mostly helical) secondary structures when dehydrated, suggestin

SUBMITTER: Yoneda JS 

PROVIDER: S-EPMC5368061 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature