PACMANS: A bioinformatically informed algorithm to predict, design, and disrupt protease-on-protease hydrolysis.

ABSTRACT: Multiple proteases in a system hydrolyze target substrates, but recent evidence indicates that some proteases will degrade other proteases as well. Cathepsin S hydrolysis of cathepsin K is one such example. These interactions may be uni- or bi-directional and change the expected kinetics. To explore potential protease-on-protease interactions in silico, a program was developed for users to input two proteases: (1) the protease-ase that hydrolyzes (2) the substrate, protease. This program identifies putative sites on the substrate protease highly susceptible to cleavage by the protease-ase, using a sliding-window approach that scores amino acid sequences by their preference in the protease-ase active site, culled from MEROPS database. We call this PACMANS, Protease-Ase Cleavage from MEROPS

SUBMITTER: Ferrall-Fairbanks MC 

PROVIDER: S-EPMC5368069 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature