Crystallographic and mutational analyses of cystathionine β-synthase in the H₂ S-synthetic gene cluster in Lactobacillus plantarum.

ABSTRACT: Cystathionine β-synthase (CBS) catalyzes the formation of l-cystathionine from l-serine and l-homocysteine. The resulting l-cystathionine is decomposed into l-cysteine, ammonia, and α-ketobutylic acid by cystathionine γ-lyase (CGL). This reverse transsulfuration pathway, which is catalyzed by both enzymes, mainly occurs in eukaryotic cells. The eukaryotic CBS and CGL have recently been recognized as major physiological enzymes for the generation of hydrogen sulfide (H₂ S). In some bacteria, including the plant-derived lactic acid bacterium Lactobacillus plantarum, the CBS- and CGL-encoding genes form a cluster in their genomes. Inactivation of these enzymes has been reported to suppress H₂ S production in bacteria; interestingly, it has been shown that H₂ S

SUBMITTER: Matoba Y 

PROVIDER: S-EPMC5368070 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature