An approach to functionally relevant clustering of the protein universe: Active site profile-based clustering of protein structures and sequences.

ABSTRACT: Protein function identification remains a significant problem. Solving this problem at the molecular functional level would allow mechanistic determinant identification-amino acids that distinguish details between functional families within a superfamily. Active site profiling was developed to identify mechanistic determinants. DASP and DASP2 were developed as tools to search sequence databases using active site profiling. Here, TuLIP (Two-Level Iterative clustering Process) is introduced as an iterative, divisive clustering process that utilizes active site profiling to separate structurally characterized superfamily members into functionally relevant clusters. Underlying TuLIP is the observation that functionally relevant families (curated by Structure-Function Linkage Database, SFLD) se

SUBMITTER: Knutson ST 

PROVIDER: S-EPMC5368075 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature