Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAG^® Technology.

ABSTRACT: Early diagnosis and treatment are crucial to the outcome of lower respiratory tract infections (LRTIs). In this study, we developed an assay combining multiplex PCR and Luminex technology (MPLT) for the detection of nine important respiratory bacterial pathogens, which frequently cause LRTIs. These were Streptococcus pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, Streptococcus pyogenes, Haemophilus influenzae, Mycoplasma pneumoniae, Legionella spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae. Through the hybridization reaction between two new synthesized multiplex PCR products and MagPlex-TAG Microspheres, we demonstrate that the detection limits for these nine pathogens were as low as 10²-10³ CFU/mL. Furtherm

SUBMITTER: Jiang L 

PROVIDER: S-EPMC5369059 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature