Exploring structural variation and gene family architecture with De Novo assemblies of 15 Medicago genomes.

ABSTRACT:

Background

Previous studies exploring sequence variation in the model legume, Medicago truncatula, relied on mapping short reads to a single reference. However, read-mapping approaches are inadequate to examine large, diverse gene families or to probe variation in repeat-rich or highly divergent genome regions. De novo sequencing and assembly of M. truncatula genomes enables near-comprehensive discovery of structural variants (SVs), analysis of rapidly evolving gene families, and ultimately, construction of a pan-genome.

Results

Genome-wide synteny based on 15 de novo M. truncatula assemblies effectively detected different types of SVs indicating that as much as 22% of the genome is involved in large structural changes, altogether affecting 28% of gene models. A total of 63

SUBMITTER: Zhou P 

PROVIDER: S-EPMC5369179 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature