Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines.

ABSTRACT:

Objective

Skeletal muscle is an important secretory organ, producing and releasing numerous myokines, which may be involved in mediating beneficial health effects of physical activity. More than 100 myokines have been identified by different proteomics approaches, but these techniques may not detect all myokines. We used mRNA sequencing as an untargeted approach to study gene expression of secreted proteins in skeletal muscle upon acute as well as long-term exercise.

Methods

Twenty-six middle-aged, sedentary men underwent combined endurance and strength training for 12 weeks. Skeletal muscle biopsies from m. vastus lateralis and blood samples were taken before and after an acute bicycle test, performed at baseline as well as after 12 weeks of training intervention. We

SUBMITTER: Pourteymour S 

PROVIDER: S-EPMC5369209 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature