The subclonal structure and genomic evolution of oral squamous cell carcinoma revealed by ultra-deep sequencing.

ABSTRACT: Recent studies suggest that head and neck squamous cell carcinomas are very heterogeneous between patients; however the subclonal structure remains unexplored mainly due to studies using only a single biopsy per patient. To deconvolute the clonal structure and describe the genomic cancer evolution, we applied whole-exome sequencing combined with ultra-deep targeted sequencing on oral squamous cell carcinomas (OSCC). From each patient, a set of biopsies was sampled from distinct geographical sites in primary tumor and lymph node metastasis.We demonstrate that the included OSCCs show a high degree of inter-patient heterogeneity but a low degree of intra-tumor heterogeneity. However, some OSCC cancers contain complex subclonal architectures comprising distinct subclones only found in geograph

SUBMITTER: Tabatabaeifar S 

PROVIDER: S-EPMC5369985 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature