Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells.

ABSTRACT: Soft tissue sarcomas with complex genomics are very heterogeneous tumors lacking simple prognosis markers or targeted therapies. Overexpression of a subset of mitotic genes from a signature called CINSARC is of bad prognosis, but the significance of this signature remains elusive. Here we precisely measure the cell cycle and mitosis duration of sarcoma cell lines and we found that the mitotic gene products overexpression does not reflect variation in the time spent during mitosis or G2/M. We also found that the CINSARC cell lines, we studied, are composed of a mixture of aneuploid, diploid, and tetraploid cells that are highly motile in vitro. After sorting diploid and tetraploid cells, we showed that the tetraploid cell clones do not possess a proliferative advantage, but are strikingly m

SUBMITTER: Jemaa M 

PROVIDER: S-EPMC5369993 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature