Genome-wide methylation analysis identifies a core set of hypermethylated genes in CIMP-H colorectal cancer.

ABSTRACT:

Background

Aberrant DNA methylation profiles are a characteristic of all known cancer types, epitomized by the CpG island methylator phenotype (CIMP) in colorectal cancer (CRC). Hypermethylation has been observed at CpG islands throughout the genome, but it is unclear which factors determine whether an individual island becomes methylated in cancer.

Methods

DNA methylation in CRC was analysed using the Illumina HumanMethylation450K array. Differentially methylated loci were identified using Significance Analysis of Microarrays (SAM) and the Wilcoxon Signed Rank (WSR) test. Unsupervised hierarchical clustering was used to identify methylation subtypes in CRC.

Results

In this study we characterized the DNA methylation profiles of 94 CRC tissues and their matched normal

SUBMITTER: McInnes T 

PROVIDER: S-EPMC5371203 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature