Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery.

ABSTRACT:

Background

Citrobacter amalonaticus Y19 is a good biocatalyst for production of hydrogen (H₂) from oxidation of carbon monoxide (CO) via the so-called water-gas-shift reaction (WGSR). It has a high H₂-production activity (23.83 mmol H₂ g^-1 cell h^-1) from CO, and can grow well to a high density on various sugars. However, its H₂-production activity is expressed only when CO is present as an inducer and in the absence of glucose.

Results

In order to avoid dependency on CO and glucose, in the present study, the native CO-inducible promoters of WGSR operons (CO dehydrogenase, CODH, and CODH-dependent hydrogenase, CO-hyd) in Y19 were carefully analyzed and replaced with strong and constitutive promote

SUBMITTER: Ainala SK 

PROVIDER: S-EPMC5371261 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature