Generation of infectious recombinant Adeno-associated virus in Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae has been successfully employed to establish model systems for a number of viruses. Such model systems are powerful tools to study the virus biology and in particular for the identification and characterization of host factors playing a role in the viral infection cycle. Adeno-associated viruses (AAV) are heavily studied due to their use as gene delivery vectors. AAV relies on other helper viruses for successful replication and on host factors for several aspects of the viral life cycle. However the role of host and helper viral factors is only partially known. Production of recombinant AAV (rAAV) vectors for gene delivery applications depends on knowledge of AAV biology and the limited understanding of host and helper viral factors may be precluding effic

SUBMITTER: Barajas D 

PROVIDER: S-EPMC5371294 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature