Tracking the Fate and Origin of Clinically Relevant Adoptively Transferred CD8⁺ T Cells In Vivo.

ABSTRACT: Adoptively transferred tumor-specific cells can mediate tumor regression in cancers refractory to conventional therapy. Autologous polyclonal tumor-specific cytotoxic T cells (CTL) generated from peripheral blood and infused into patients with metastatic melanoma show enhanced persistence, compared to equivalent numbers of more extensively expanded monoclonal CTL, and are associated with complete remissions (CR) in select patients. We applied high-throughput T cell receptor Vβ sequencing (HTTCS) to identify individual clonotypes within CTL products, track them in vivo post-infusion and then deduce the pre-adoptive transfer (endogenous) frequencies of cells ultimately responsible for tumor regression. The summed in vivo post-transfer frequencies of the top 25 HTTCS-defined clo

SUBMITTER: Chapuis AG 

PROVIDER: S-EPMC5371351 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature