CdiA Effectors Use Modular Receptor-Binding Domains To Recognize Target Bacteria.

ABSTRACT: Contact-dependent growth inhibition (CDI) systems encode CdiA effectors, which bind to specific receptors on neighboring bacteria and deliver C-terminal toxin domains to suppress target cell growth. Two classes of CdiA effectors that bind distinct cell surface receptors have been identified, but the molecular basis of receptor specificity is not understood. Alignment of BamA-specific CdiA^EC93 from Escherichia coli EC93 and OmpC-specific CdiA^EC536 from E. coli 536 suggests that the receptor-binding domain resides within a central region that varies between the two effectors. In support of this hypothesis, we find that CdiA^EC93 fragments containing residues Arg1358 to Phe1646 bind specifically to purified BamA. Moreover, chimeric CdiA^EC93</

SUBMITTER: Ruhe ZC 

PROVIDER: S-EPMC5371414 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature