Chromosomal aneuploidies induced upon Lamin B2 depletion are mislocalized in the interphase nucleus.

ABSTRACT: Chromosome territories assume non-random positions in the interphase nucleus with gene-rich chromosomes localized toward the nuclear interior and gene-poor chromosome territories toward the nuclear periphery. Lamins are intermediate filament proteins of the inner nuclear membrane required for the maintenance of nuclear structure and function. Here, we show using whole-genome expression profiling that Lamin A/C or Lamin B2 depletion in an otherwise diploid colorectal cancer cell line (DLD1) deregulates transcript levels from specific chromosomes. Further, three-dimensional fluorescence in situ hybridization (3D-FISH) analyses of a subset of these transcriptionally deregulated chromosome territories revealed that the diploid chromosome territories in Lamin-depleted cells largely maintain con

SUBMITTER: Ranade D 

PROVIDER: S-EPMC5371638 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature