Experimental Evolution of Escherichia coli Harboring an Ancient Translation Protein.

ABSTRACT: The ability to design synthetic genes and engineer biological systems at the genome scale opens new means by which to characterize phenotypic states and the responses of biological systems to perturbations. One emerging method involves inserting artificial genes into bacterial genomes and examining how the genome and its new genes adapt to each other. Here we report the development and implementation of a modified approach to this method, in which phylogenetically inferred genes are inserted into a microbial genome, and laboratory evolution is then used to examine the adaptive potential of the resulting hybrid genome. Specifically, we engineered an approximately 700-million-year-old inferred ancestral variant of tufB, an essential gene encoding elongation factor Tu, and inserted it in a mo

SUBMITTER: Kacar B 

PROVIDER: S-EPMC5371648 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature