Efficient and Robust Paramyxoviridae Reverse Genetics Systems.

ABSTRACT: The notoriously low efficiency of Paramyxoviridae reverse genetics systems has posed a limiting barrier to the study of viruses in this family. Previous approaches to reverse genetics have utilized a wide variety of techniques to overcome the technical hurdles. Although robustness (i.e., the number of attempts that result in successful rescue) has been improved in some systems with the use of stable cell lines, the efficiency of rescue (i.e., the proportion of transfected cells that yield at least one successful rescue event) has remained low. We have substantially increased rescue efficiency for representative viruses from all five major Paramyxoviridae genera (from ~1 in 10⁶-10⁷ to ~1 in 10²-10³ transfected cells) by the addition

SUBMITTER: Beaty SM 

PROVIDER: S-EPMC5371697 | biostudies-literature | 2017 Mar-Apr

REPOSITORIES: biostudies-literature