Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd).

ABSTRACT:

Background

Biologically, hydrogen (H₂) can be produced through dark fermentation and photofermentation. Dark fermentation is fast in rate and simple in reactor design, but H₂ production yield is unsatisfactorily low as <4 mol H₂/mol glucose. To address this challenge, simultaneous production of H₂ and ethanol has been suggested. Co-production of ethanol and H₂ requires enhanced formation of NAD(P)H during catabolism of glucose, which can be accomplished by diversion of glycolytic flux from the Embden-Meyerhof-Parnas (EMP) pathway to the pentose-phosphate (PP) pathway in Escherichia coli. However, the disruption of pgi (phosphoglucose isomerase) for complete diversion of carbon flux to the PP p

SUBMITTER: Sundara Sekar B 

PROVIDER: S-EPMC5372246 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature