Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood.

ABSTRACT:

Background

Genetics explains a small proportion of variance in body mass index at the population level. Epigenetics, commonly measured by gene methylation, holds promise for understanding obesity risk factors and mechanisms.

Methods

Participants were 431 adolescents aged 10-15 years. BMI z-score, waist circumference z-score, and percent body fat were measured. Saliva samples were collected and methylation of promoter regions of four candidate genes or sequences (LEP, ICAM-1, CRH, and LINE-1) were measured in 3-4 CpG sites each. Linear regression was used to identify associations of methylation with obesity-related outcomes.

Results

After adjusting for age, in sex-stratified analysis, the three obesity-related outcomes were negatively associated wi

SUBMITTER: Dunstan J 

PROVIDER: S-EPMC5372250 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature