Anti-tumor effects of everolimus and metformin are complementary and glucose-dependent in breast cancer cells.

ABSTRACT:

Background

Clinical efficacy of the mTOR inhibitor everolimus is limited in breast cancer and regularly leads to side-effects including hyperglycemia. The AMPK inhibitor and anti-diabetic drug metformin may counteract everolimus-induced hyperglycemia, as well as enhancing anti-cancer efficacy. We investigated the glucose-dependent growth-inhibitory properties of everolimus, metformin and the combination in breast cancer cell lines.

Methods

The breast cancer cell lines MCF-7, MDA-MB-231 and T47D were cultured in media containing 11 mM or 2.75 mM glucose with 21% or 1% oxygen. Everolimus and metformin treated cells were subjected to cytotoxicity and clonogenic assays, western blotting, FACS and metabolic measurements.

Results

Everolimus was less effective in MCF7 cells

SUBMITTER: Ariaans G 

PROVIDER: S-EPMC5372253 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature