A deletion in the intergenic region upstream of Ednrb causes head spot in the rat strain KFRS4/Kyo.

ABSTRACT:

Background

Head spot is one of the phenotypes identified in the KFRS4/Kyo rat strain. Although previous linkage analysis suggested that Ednrb, which is frequently involved in coat color variations in various animals, could be the gene responsible for this phenotype, no mutations have been identified in its coding region.

Results

To identify mutations causative of this phenotype in KFRS4/Kyo, we analyzed target capture sequencing data that we recently generated. Our target capture method has a unique feature, i.e., it covers not only exonic regions but also conserved non-coding sequences (CNSs) among vertebrates; therefore, it has the potential to detect regulatory mutations. We identified a deletion of approximately 50 kb in length approximately 50 kb upstream of Ednrb. A co

SUBMITTER: Yoshihara M 

PROVIDER: S-EPMC5372274 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature