Amplification and up-regulation of MIR30D was associated with disease progression of cervical squamous cell carcinomas.

ABSTRACT:

Background

Cervical squamous cell carcinoma (CSCC) is the most frequent type among cervical cancers. Although the altered miRNA miR-30d expression and the amplified chromosome locus of MIR30D, 8q24, have been reported in somatic cancers, the definitive functional impact of such region especially in CSCC remains under-investigated.

Methods

One hundred thirty-six cases of CSCC tissues and matched adjacent normal ovarian epithelial tissues were assessed in this study. FISH and qPCR were performed to detect the copy number and microRNA expression of MIR30D gene in the collected samples. In in-vitro study, proliferation of CSCC cells were analyzed using WST-1 assay and invasion abilities of CSCC cells were evaluated by transwell assay. In-vivo study using a model of nude mice bea

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC5372318 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature