Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs.

ABSTRACT: Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the tran

SUBMITTER: Su LJ 

PROVIDER: S-EPMC5372358 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature