Optimizing conditions for calcium phosphate mediated transient transfection.

ABSTRACT:

Background

Calcium phosphate mediated transfection has been used for delivering DNA into mammalian cells in excess of 30 years due to its most low cost for introducing recombinant DNA into culture cells. However, multiple factors affecting the transfect efficiency are commonly recognized meanwhile for years, the low transfection efficiency of this approach on higher differentiated and non-tumor cells such as CHO and C2C12 limits its application on research.

Results

In this paper, we systematically evaluated the possible factors affecting the transfection rate of this approach. Two categories, calcium phosphate-DNA co-precipitation and on-cell treatments were set for optimization of plasmid DNA transfection into CHO and C2C12 cell-lines. Throughout experimentation of these ca

SUBMITTER: Guo L 

PROVIDER: S-EPMC5372392 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature