Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance.

ABSTRACT: Understanding the interplay between molecules and lipid membranes is fundamental when studying cellular and biotechnological phenomena. Partition between aqueous media and lipid membranes is key to the mechanism of action of many biomolecules and drugs. Quantifying membrane partition, through adequate and robust parameters, is thus essential. Surface Plasmon Resonance (SPR) is a powerful technique for studying 1:1 stoichiometric interactions but has limited application to lipid membrane partition data. We have developed and applied a novel mathematical model for SPR data treatment that enables determination of kinetic and equilibrium partition constants. The method uses two complementary fitting models for association and dissociation sensorgram data. The SPR partition data obtained for th

SUBMITTER: Figueira TN 

PROVIDER: S-EPMC5372468 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature