The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins-Metal Complexes.

ABSTRACT: The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly co

SUBMITTER: Merlos Rodrigo MA 

PROVIDER: S-EPMC5372626 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature