Efficient Differentiation of TBX18⁺/WT1⁺ Epicardial-Like Cells from Human Pluripotent Stem Cells Using Small Molecular Compounds.

ABSTRACT: The epicardium promotes neovascularization and cardiomyocyte regeneration by generating vascular smooth muscle cells (SMCs) and producing regenerative factors after adult heart infarction. It is therefore a potential cell resource for repair of the injured heart. However, the epicardium also participates in fibrosis and scarring of the injured heart, complicating its use in regenerative medicine. In this study, we report coexpression of TBX18 and WT1 in the majority of epicardial cells during mouse embryonic epicardial development. Furthermore, we describe a convenient chemically defined, immunogen-free, small molecule-based method for generating TBX18⁺/WT1⁺ epicardial-like cell populations with 80% homogeneity from human pluripotent stem cells by modulation of the WN

SUBMITTER: Zhao J 

PROVIDER: S-EPMC5372775 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature