Evaluating multi-locus phylogenies for species boundaries determination in the genus Diaporthe.

ABSTRACT:

Background

Species identification is essential for controlling disease, understanding epidemiology, and to guide the implementation of phytosanitary measures against fungi from the genus Diaporthe. Accurate Diaporthe species separation requires using multi-loci phylogenies. However, defining the optimal set of loci that can be used for species identification is still an open problem.

Methods

Here we addressed that problem by identifying five loci that have been sequenced in 142 Diaporthe isolates representing 96 species: TEF1, TUB, CAL, HIS and ITS. We then used every possible combination of those loci to build, analyse, and compare phylogenetic trees.

Results

As expected, species separation is better when all five loci

SUBMITTER: Santos L 

PROVIDER: S-EPMC5372842 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature