Alterations in airway microbiota in patients with PaO2/FiO2 ratio ≤ 300 after burn and inhalation injury.

ABSTRACT:

Background

Injury to the airways after smoke inhalation is a major mortality risk factor in victims of burn injuries, resulting in a 15-45% increase in patient deaths. Damage to the airways by smoke may induce acute respiratory distress syndrome (ARDS), which is partly characterized by hypoxemia in the airways. While ARDS has been associated with bacterial infection, the impact of hypoxemia on airway microbiota is unknown. Our objective was to identify differences in microbiota within the airways of burn patients who develop hypoxemia early after inhalation injury and those that do not using next-generation sequencing of bacterial 16S rRNA genes.

Results

DNA was extracted from therapeutic bronchial washings of 48 patients performed within 72 hours of hospitalization for burn

SUBMITTER: Walsh DM 

PROVIDER: S-EPMC5373524 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature