Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography.

ABSTRACT: Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants. In order to assess the possible health effects of milk EV, it is essential to use isolates of high purity from other more abundant milk structures with well-documented bioactive properties. Furthermore, gentle isolation procedures are important for reducing the risk of generating vesicle artefacts, particularly when EV subpopulations are investigated. In this study, we present two isolation approaches accomplished in three steps based on size-exclusion chromatography (SEC) resulting in effective and reproducible EV isolation from raw milk. The approaches do not require any EV pelleting and can be applied to b

SUBMITTER: Blans K 

PROVIDER: S-EPMC5373680 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature