Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes.

ABSTRACT: Diverse cellular signaling events, including B cell receptor (BCR) activation, are hypothesized to be facilitated by domains enriched in specific plasma membrane lipids and proteins that resemble liquid-ordered phase-separated domains in model membranes. This concept remains controversial and lacks direct experimental support in intact cells. Here, we visualize ordered and disordered domains in mouse B lymphoma cell membranes using super-resolution fluorescence localization microscopy, demonstrate that clustered BCR resides within ordered phase-like domains capable of sorting key regulators of BCR activation, and present a minimal, predictive model where clustering receptors leads to their collective activation by stabilizing an extended ordered domain. These results provide evidence for t

SUBMITTER: Stone MB 

PROVIDER: S-EPMC5373823 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature