Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue.

ABSTRACT: In transplantation, there is a critical need for noninvasive biomarker platforms for monitoring immunologic rejection. We hypothesized that transplanted tissues release donor-specific exosomes into recipient circulation and that the quantitation and profiling of donor intra-exosomal cargoes may constitute a biomarker platform for monitoring rejection. Here, we have tested this hypothesis in a human-into-mouse xenogeneic islet transplant model and validated the concept in clinical settings of islet and renal transplantation. In the xenogeneic model, we quantified islet transplant exosomes in recipient blood over long-term follow-up using anti-HLA antibody, which was detectable only in xenoislet recipients of human islets. Transplant islet exosomes were purified using anti-HLA antibody-conju

SUBMITTER: Vallabhajosyula P 

PROVIDER: S-EPMC5373894 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature