Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans.

ABSTRACT: Understanding the rearrangement of gas-phase ions via tandem mass spectrometry is critical to improving manual and automated interpretation of complex datasets. N-glycan analysis may be carried out under collision induced (CID) or higher energy collision dissociation (HCD), which favors cleavage at the glycosidic bond. However, fucose migration has been observed in tandem MS, leading to the formation of new bonds over four saccharide units away. In the following work, we report the second instance of saccharide migration ever to occur for N-glycans. Using horseradish peroxidase as a standard, the beta-1,2 xylose was observed to migrate from a hexose to a glucosamine residue on the (Xyl)Man₃GlcNac₂ glycan. This investigation was followed up in a complex N-linked glycan

SUBMITTER: Hecht ES 

PROVIDER: S-EPMC5373971 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature