Parkin-mediated mitophagy is downregulated in browning of white adipose tissue.

ABSTRACT:

Objective

Browning of white adipose tissue (WAT) promotes increased energy expenditure through the action of uncoupling protein 1 (UCP1) and is an attractive target to promote weight loss in obesity. Lowering of mitochondrial membrane potential by UCP1 is uniquely beneficial in this context; in other tissues, reduced membrane potential promotes mitochondrial clearance via mitophagy. It is unknown how parkin-mediated mitophagy is regulated in beige adipocytes.

Methods

The relationship between parkin expression and WAT browning was investigated in 3T3-L1 adipocytes and parkin-deficient male C57BL/6 mice in response to pharmacological browning stimuli.

Results

Rosiglitazone treatment in 3T3-L1 adipocytes promoted mitochondrial biogenesis, UCP1 expression, and mitochondri

SUBMITTER: Taylor D 

PROVIDER: S-EPMC5373982 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature