Phylogenetic Heatmaps Highlight Composition Biases in Sequenced Reads.

ABSTRACT: Due to advancements in sequencing technology, sequence data production is no longer a constraint in the field of microbiology and has made it possible to study uncultured microbes or whole environments using metagenomics. However, these new technologies introduce different biases in metagenomic sequencing, affecting the nucleotide distribution of resulting sequence reads. Here, we illustrate such biases using two methods. One is based on phylogenetic heatmaps (PGHMs), a novel approach for compact visualization of sequence composition differences between two groups of sequences containing the same phylogenetic groups. This method is well suited for finding noise and biases when comparing metagenomics samples. We apply PGHMs to detect noise and bias in the data produced with different DNA ex

SUBMITTER: Choudhari S 

PROVIDER: S-EPMC5374381 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature