AfterQC: automatic filtering, trimming, error removing and quality control for fastq data.

ABSTRACT:

Background

Some applications, especially those clinical applications requiring high accuracy of sequencing data, usually have to face the troubles caused by unavoidable sequencing errors. Several tools have been proposed to profile the sequencing quality, but few of them can quantify or correct the sequencing errors. This unmet requirement motivated us to develop AfterQC, a tool with functions to profile sequencing errors and correct most of them, plus highly automated quality control and data filtering features. Different from most tools, AfterQC analyses the overlapping of paired sequences for pair-end sequencing data. Based on overlapping analysis, AfterQC can detect and cut adapters, and furthermore it gives a novel function to correct wrong bases in the overlapping regions. An

SUBMITTER: Chen S 

PROVIDER: S-EPMC5374548 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature