Increased Kruppel-like factor 12 in recurrent implantation failure impairs endometrial decidualization by repressing Nur77 expression.

ABSTRACT:

Background

Decidualization is a prerequisite for successful implantation and the establishment of pregnancy. A critical role of impaired decidualization in subfertility has been established. In human endometrial stromal cells (hESCs), Krüppel-like factor 12 (KLF12) and Nur77 are novel regulators of decidualization. We investigated whether KLF12 impaired the decidualization of hESCs in recurrent implantation failure (RIF) patients.

Methods

Endometrial tissues and hESCs were collected from RIF patients (n = 34) and fertile controls (n = 30) for in vitro analysis. Primary hESCs isolated from RIF endometrial tissues were used to evaluate the biological functions of KLF12 and Nur77. In addition, their molecular mechanisms were investigated by adenovirus-mediated overexpression. G

SUBMITTER: Huang C 

PROVIDER: S-EPMC5374626 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature