Gramicidin A Channel Formation Induces Local Lipid Redistribution I: Experiment and Simulation.

ABSTRACT: Integral membrane protein function can be modulated by the host bilayer. Because biological membranes are diverse and nonuniform, we explore the consequences of lipid diversity using gramicidin A channels embedded in phosphatidylcholine (PC) bilayers composed of equimolar mixtures of di-oleoyl-PC and di-erucoyl-PC (dC_18:1+dC_22:1, respectively), di-palmitoleoyl-PC and di-nervonoyl-PC (dC_16:1+dC_24:1, respectively), and di-eicosenoyl-PC (pure dC_20:1), all of which have the same average bilayer chain length. Single-channel lifetime experiments, molecular dynamics simulations, and a simple lipid compression model are used in tandem to gain insight into lipid redistribution around the channel, which partially alleviates the bilayer deformati

SUBMITTER: Beaven AH 

PROVIDER: S-EPMC5375002 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature