Differential Regulation of Macrophage Glucose Metabolism by Macrophage Colony-stimulating Factor and Granulocyte-Macrophage Colony-stimulating Factor: Implications for ¹⁸F FDG PET Imaging of Vessel Wall Inflammation.

ABSTRACT: Purpose To determine the divergence of immunometabolic phenotypes of macrophages stimulated with macrophage colony-stimulating factor (M-CSF) and granulocyte-M-CSF (GM-CSF) and its implications for fluorine 18 (¹⁸F) fluorodeoxyglucose (FDG) imaging of atherosclerosis. Materials and Methods This study was approved by the animal care committee. Uptake of 2-deoxyglucose and various indexes of oxidative and glycolytic metabolism were evaluated in nonactivated murine peritoneal macrophages (MΦ₀) and macrophages stimulated with M-CSF (MΦ_M-CSF) or GM-CSF (MΦ_GM-CSF). Intracellular glucose flux was measured by using stable isotope tracing of glycolytic and tricyclic acid intermediary metabolites. ¹⁸F-FDG uptake was evaluated in murine atheroscl

SUBMITTER: Tavakoli S 

PROVIDER: S-EPMC5375627 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature