17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes.

ABSTRACT:

Background

The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol.

Methods

The skeletal muscle cell line C2C12 was treated with 17β-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA levels of myogenic regulatory factors, myosin heavy chain, oxytocin, oxytocin receptor and adipogenic factors were analysed in C2C12 myotubes.

Results

It was demonstrated that C2C12 myoblasts and myotubes express oxytocin and its receptor, in particular the receptor levels physiologically increase in differentiated myotubes. Myotubes treated with 17β-estradio

SUBMITTER: Berio E 

PROVIDER: S-EPMC5376115 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature